WebSep 9, 2024 · osteosarcoma cells (HOS, ATCC CRL-1543), following the methodology previously described in [9,10]. The HOS cells were kept in RPMI supplemented with 10% fetal bovine serum and 1% penicillin / streptomycin in an atmosphere of 5% CO2, 95% humidity and a temperature of 37°C. Cells were sub-cultured once they had a 90% cell confluency … WebApr 7, 2024 · The osteosarcoma cell line (143B, ATCC: CRL-8303, and HOS, ATCC: HTB-96TM, were purchased from the American Type Culture Collection) were cultured in DMEM/F-12 medium (DMEM, Gibco, Shanghai, China) containing 10% fetal bovine serum (Gibco, American), and 100 µg/mL streptomycin and 100 U/mL penicillin (Solarbio, Beijing, …
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Webhos ( ATCC ) ATCC is a verified supplier ATCC manufactures this product About News Press Release Team Advisors Partners Contact Bioz Stars Bioz vStars 86 Buy from Supplier … WebHuman Osteosarcoma Cells (HOS, ATCC, CRL 1543) were cul- tured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma, USA), supplemented with 10% Fetal Calf Serum (FCS), 1% non- essential amino acids, 150 µg/ml ascorbic acid, 0.02 mM L-glu- tamine, 0.01 M HEPES (4-(2-hydroxyethyl)-1-piperazineethane- sulfonic acid), penicillin (100 units/ml) and … mesh basketball shorts public exhibition
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WebL. Fassina, ... S. Van Vlierberghe, in Biomaterials for Bone Regeneration, 2014 15.2.2 Cell seeding. The human osteosarcoma cell line SAOS-2 (sarcoma osteogenic-2) was obtained from the American Type Culture Collection (HTB85, ATCC). The cells were cultured in McCoy’s 5A modified medium with L-glutamine and HEPES (4-(2-hydroxyethyl)piperazine … WebAug 11, 2024 · 293T, MT4 (RRID:CVCL_2632), HeLa, MAGIC5 (HeLa derivative [Mochizuki et al., 1999]), and HOS cells were maintained under standard conditions. Cells were originally obtained from ATCC (except MAGIC5 cells) and routinely tested negative for mycoplasma contamination (PCR Mycoplasma Detection kit, Takara). WebAntibodies & Protein Biology Antibodies & Protein Biology Antibody Production and Purification Electrophoresis, Western Blotting and ELISA Precast Electrophoresis Gels Primary Antibodies Secondary Antibodies View All Antibodies Biochemicals and Reagents Biological Buffers Custom Services and Products Enzymes and Inhibitors View All Protein … mesh basketball shorts with tape