Hur antibody

Author: jpub

August undefined, 2024

WebThe in-frame internal tandem duplication (ITD) of the FLT3 gene is an important negative prognostic factor in acute myeloid leukemia (AML). FLT3-ITD is constitutive active and partially retained in the endoplasmic reticulum (ER). Recent reports show that 3′UTRs function as scaffolds that can regulate the localization of plasma membrane proteins by …

Circular RNA 0001073 Attenuates Malignant Biological Behaviours …

Web10 feb. 2011 · Using a phospho-Ser 318-specific HuR antibody, we found a strong increase in nuclear HuR phosphorylation in DLD-1 cells when compared with nontransformed CCD 841 colon epithelial cells. Importantly, a strong increase in HuR phosphorylation at Ser 318 was also found in tissue specimen from human colon carcinomas. WebInvitrogen Anti-HuR Monoclonal (3A2), Catalog # MA1-167. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunoprecipitation (IP) and RNA Immunoprecipitation … clevergene bangalore

ADORA2A-AS1 Restricts Hepatocellular Carcinoma Progression

Web1 aug. 2011 · Anti-HuR antibody (Left) and anti-HuB antibody (Right) were used. U1 70 K was used as a loading control. β-tubulin III is a neuronal marker. ( E ) CoIP was carried out with nonspecific IgG or antibodies against hypophosphorylated RNAPII (8WG16), ser-2 phosphorylated RNAPII (H5), or ser-5 phosphorylated RNAPII (H14) using protein lysate … WebAntibody Dilution Buffer (1X PBS / 1% BSA / 0.3% Triton X-100): To prepare 10 ml, add 30 µl Triton™ X-100 to 10 ml 1X PBS. Mix well then add 0.1 g BSA , mix. Recommended … WebAnti-Hu antibody (also known as antineuronal nuclear antibody-type 1, ANNA-1) was the first recognized autoantibody marker of small cell lung cancer (Graus et al., 1985). In a previous study of 162 consecutive patients with anti-Hu antibodies, malignancy was present in 88% of patients, being small cell lung cancer in 93% (Lucchinetti et al., 1998). clever gear \\u0026 other smart innovations

Identification of a target RNA motif for RNA-binding protein HuR

WebHuR Antibody (3A2) is an IgG 1 κ mouse monoclonal HuR antibody (also designated ELAV like RNA binding protein 4 antibody or ELAVL4 antibody) suitable for the … Web10 jan. 2024 · Human antigen R (HuR) is an essential regulator of RNA metabolism, but its function in metabolism remains unclear. This study identifies HuR as a major repressor during adipogenesis. Knockdown... clever gear \u0026 other smart innovationsWebApplications. This RIPAb+ HuR -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers. RIP lysate from MCF7 cells (2 X 10 6 cell equivalents per IP) was subjected to immunoprecipitation using 0.5 μg of either a normal rabbit IgG or Anti-HuR antibody. bms subject list

"WebAnti-HuR antibody was used in the IP ubiquitination assay. MG132-treated HCT116 cells were collected and incubated with Protein-A/G MagBeads (Yeason, Shanghai, China) and antibody. After overnight incubation, the immune complexes were centrifuged and washed, and the proteins were detected by Western blotting with an anti-ubiquitin antibody … " - Hur antibody

Hur antibody

HuR-hnRNP interactions and the effect of cellular stress

WebHuR antibody (CL488-66549) Proteintech CoraLite® Plus 488-conjugated HuR Monoclonal antibody HuR Monoclonal Antibody for IF Host / Isotype Mouse / IgG2a … WebAll lanes : Anti-HuR / ELAVL1 antibody (ab28660) at 1 µg/ml Lane 1 : Wild-type SW480 cell lysate Lane 2 : ELAVL1 knockout SW480 cell lysate Lane 3 : HEK-293 cell lysate Lane 4 …

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WebDescription. HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA bindnig protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. WebWe found that an mRNA-binding protein, human antigen R (HuR) associated with the 3′ UTR of AREG mRNA in response to UVB exposure, leading to enhanced mRNA stabilization. We also evaluated the …

Web21 mrt. 2024 · HUR 3 4 5 ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) 2 3 Embryonic Lethal, Abnormal Vision, Drosophila, Homolog-Like 1 2 3 … WebHuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA bindnig protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene …

WebHuR antibody (CL488-66549) Proteintech CoraLite® Plus 488-conjugated HuR Monoclonal antibody HuR Monoclonal Antibody for IF Host / Isotype Mouse / IgG2a Reactivity Human, mouse, rat Applications IF Conjugate CoraLite® Plus 488 Fluorescent Dye CloneNo. 1A11H2 Review Product Cat no : CL488-66549 Synonyms WebIHC was performed as previously described. 29 After incubation with anti-Cleaved Caspase-3 or anti-HuR antibodies (#12,582) overnight at 4 °C, the samples were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies for 10 min at 37 °C. All imaged were obtained using an upright microscope (BX53, Olympus, Japan).

WebHuR inhibitor KH-3 is a small molecule, selective inhibitior of HuR function with Ki of 0.83 and 0.72 uM in FP and AlphaLISA assays, respectively. KH-3 potently inhibits breast cancer cell growth in vitro and in vivo. KH-3 inhibits breast cancer cell invasion in vitro as well as delays initiation of lung colonies and improves mouse survival in ...

Web7 feb. 2024 · An HRP-conjugated alpha tubulin antibody (dilution 1:2,000, Rockland, Hercules, CA, USA) was used as a loading control. Signals on the membranes were detected using chemiluminescence (ChemiDoc MP, Bio-Rad). Protein bands were quantified using ImageJ software with the Band/Peak Quantification Tool. 1 2.3. Design of the in … clever geheizt backnangWebInvitrogen Anti-HuR Monoclonal (3A2), Catalog # 39-0600. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (IHC), ELISA (ELISA) and RNA … cleverget 10.0.0.0 crackWebHuR Antibody (A-21277) in IHC Molecular and cellular biology 2024 - Published figure using HuR monoclonal antibody (Product # A-21277) in Immunohistochemistry … clever georgia cyberWeb7 apr. 2024 · In 2015 and 2016, continued efforts to develop highly multiplexed FISH methods increased the number of detected genes from a dozen to several hundred and then up to one thousand transcripts per cell [multiplexed error-robust FISH (MERFISH) [] and sequential FISH (seqFISH), [] Figure 1a,b].To encode as many transcripts as possible, … bms subjects sem 1WebWe determined that inhibitors of HuR (ELAVL1), a prosurvival mRNA stability factor that regulates cyclin D1, and an inhibitor of Yes … clevergeheizt matthias schaafWeb25 However, although HuR acts as a positive regulator, CUGBP1 acts as a negative regulator of TJ/AJ proteins by competing with HuR for binding to the 3 0 UTR of occludin and E-cadherin. 22 In our ... clevergesund.comWeb1 dag geleden · The following primary antibodies were used for immunoblotting: antibodies against HA Tag (3724, CST), HuR (2582, CST), and pERK1/2 (4370, CST) were used at 1:1,000 dilution, and antibodies against Vinculin (13901, CST) and Tubulin (T6199, Sigma) were used at 1:3,000 dilution as the internal controls. clever geneva county